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l lab scale bioreactors  (Eppendorf AG)


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    Structured Review

    Eppendorf AG l lab scale bioreactors
    Effect of lysE deletion on 5-aminovaleramide production in C. glutamicum. Strains expressing Ptuf davB either episomally in the basic lysine producer LYS-1 (A) or genomically in the advanced producer LYS-12 (B) were compared with their respective lysE-deficient derivatives. The data represent the yields for lysine and 5-AVD and the specific growth rate. Cultivations were performed in a miniaturized <t>bioreactor</t> system with glucose minimal medium. Data represent mean ± standard error from three biological replicates
    L Lab Scale Bioreactors, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 96/100, based on 512 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/l+lab+scale+bioreactors/Scale/pmc12930585-413-5-17
    Average 96 stars, based on 512 article reviews
    l lab scale bioreactors - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer"

    Article Title: Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer

    Journal: Microbial Cell Factories

    doi: 10.1186/s12934-026-02922-1

    Effect of lysE deletion on 5-aminovaleramide production in C. glutamicum. Strains expressing Ptuf davB either episomally in the basic lysine producer LYS-1 (A) or genomically in the advanced producer LYS-12 (B) were compared with their respective lysE-deficient derivatives. The data represent the yields for lysine and 5-AVD and the specific growth rate. Cultivations were performed in a miniaturized bioreactor system with glucose minimal medium. Data represent mean ± standard error from three biological replicates
    Figure Legend Snippet: Effect of lysE deletion on 5-aminovaleramide production in C. glutamicum. Strains expressing Ptuf davB either episomally in the basic lysine producer LYS-1 (A) or genomically in the advanced producer LYS-12 (B) were compared with their respective lysE-deficient derivatives. The data represent the yields for lysine and 5-AVD and the specific growth rate. Cultivations were performed in a miniaturized bioreactor system with glucose minimal medium. Data represent mean ± standard error from three biological replicates

    Techniques Used: Expressing

    Related Articles

    Control:

    Article Title: Multi-omics view of recombinant Yarrowia lipolytica: Enhanced ketogenic amino acid catabolism increases polyketide-synthase-driven docosahexaenoic production to high selectivity at the gram scale.
    Article Snippet: .. The performance of DHA production was evaluated in 1 L lab-scale bioreactors which were monitored and controlled using DASGIP control software (SR0700ODLS, Eppendorf, Hamburg, Germany). ..

    Article Title: Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer
    Article Snippet: .. Strains were grown in 1 L lab-scale bioreactors, monitored and controlled using the DASGIP control software (SR0700ODLS, Eppendorf, Hamburg, Germany). .. The initial batch medium (300 mL) contained: 70 g L −1 sugar beet molasses (Pfeifer & Langen, Köln, Germany), 50 g L −1 sucrose, 15 g L −1 yeast extract (Becton Dickinson), 10 mL L −1 corn steep liquor (Selfmade-Baits, Brandenburg, Germany), 35 g L −1 (NH 4 ) 2 SO 4 , 2 g L −1 citric acid, 1.25 g L −1 KH 2 PO 4 , 1.25 g L −1 Na 2 HPO 4 , 1.25 g L −1 MgSO 4 ·7H 2 O, 35 mg L −1 FeSO 4 ·7 H 2 O, 35 mg L −1 FeCl 3 ·6H 2 O, 30 mg L −1 of ZnSO 4 ·7H 2 O, 168 mg L −1 of CaSO 4 ·2H 2 O, 9.1 mg L −1 of MnSO 4 ·H 2 O, 15 mL of a vitamin solution (300 mg L −1 biotin, 500 mg L −1 thiamin·HCl, 600 mg L −1 nicotinamide, 2 g L −1 calcium pantothenate), 1.43 mL of a trace element solution (2.1 g L −1 citric acid, 300 mg L −1 boric acid 456 mg L −1 CuSO 4 ·5H 2 O, 428 mg L −1 CoSO 4 ·7H 2 O, 338 mg L −1 NiSO 4 ·6H 2 O, and 59 mg L −1 Na 2 MoO 4 ·2H 2 O) and 2 mL L −1 antifoam.

    Article Title: Systems metabolic engineering upgrades Corynebacterium glutamicum for selective high-level production of the chiral drug precursor and cell-protective extremolyte L-pipecolic acid.
    Article Snippet: .. The performance of L-pipecolic acid-producing strains was evaluated in a fed-batch process performed in 1 L lab-scale bioreactors, monitored, and controlled using DASGIP control software (SR0700ODLS, Eppendorf, Hamburg, Germany). .. The batch medium (300 mL) contained, per liter: 85 g of glucose, 25 g of (NH4)2SO4, 15 g of yeast extract (Becton Dickinson), 2 g of citric acid, 1.25 g of KH2PO4, 1.25 g of Na2HPO4, 1.25 g of MgSO4 ⋅ 7 H2O, 168 mg of CaSO4 ⋅ 2 H2O, 70 mg of FeSO4 ⋅ 7 H2O, 30 mg of ZnSO4 ⋅ 7 H2O, 9.1 mg of MnSO4 ⋅ H2O, 15 mL of vitamin solution (300 mg L− 1 biotin, 500 mg L− 1 thiamin ⋅ HCl, 600 mg L− 1 nicotinamide, 2 g L− 1 calcium pantothenate), and 1.43 mL of a trace element solution (2.1 g L− 1 citric acid, 228 mg L− 1 CuSO4 ⋅ 5 H2O, 214 mg L− 1 CoSO4 ⋅ 7 H2O, 169 mg L− 1 NiSO4 ⋅ 6 H2O, 150 mg L− 1 Na2B4O7⋅10H2O, and 29.5 mg L− 1 Na2MoO4 ⋅ 2 H2O).

    Software:

    Article Title: Multi-omics view of recombinant Yarrowia lipolytica: Enhanced ketogenic amino acid catabolism increases polyketide-synthase-driven docosahexaenoic production to high selectivity at the gram scale.
    Article Snippet: .. The performance of DHA production was evaluated in 1 L lab-scale bioreactors which were monitored and controlled using DASGIP control software (SR0700ODLS, Eppendorf, Hamburg, Germany). ..

    Article Title: Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer
    Article Snippet: .. Strains were grown in 1 L lab-scale bioreactors, monitored and controlled using the DASGIP control software (SR0700ODLS, Eppendorf, Hamburg, Germany). .. The initial batch medium (300 mL) contained: 70 g L −1 sugar beet molasses (Pfeifer & Langen, Köln, Germany), 50 g L −1 sucrose, 15 g L −1 yeast extract (Becton Dickinson), 10 mL L −1 corn steep liquor (Selfmade-Baits, Brandenburg, Germany), 35 g L −1 (NH 4 ) 2 SO 4 , 2 g L −1 citric acid, 1.25 g L −1 KH 2 PO 4 , 1.25 g L −1 Na 2 HPO 4 , 1.25 g L −1 MgSO 4 ·7H 2 O, 35 mg L −1 FeSO 4 ·7 H 2 O, 35 mg L −1 FeCl 3 ·6H 2 O, 30 mg L −1 of ZnSO 4 ·7H 2 O, 168 mg L −1 of CaSO 4 ·2H 2 O, 9.1 mg L −1 of MnSO 4 ·H 2 O, 15 mL of a vitamin solution (300 mg L −1 biotin, 500 mg L −1 thiamin·HCl, 600 mg L −1 nicotinamide, 2 g L −1 calcium pantothenate), 1.43 mL of a trace element solution (2.1 g L −1 citric acid, 300 mg L −1 boric acid 456 mg L −1 CuSO 4 ·5H 2 O, 428 mg L −1 CoSO 4 ·7H 2 O, 338 mg L −1 NiSO 4 ·6H 2 O, and 59 mg L −1 Na 2 MoO 4 ·2H 2 O) and 2 mL L −1 antifoam.

    Article Title: Systems metabolic engineering upgrades Corynebacterium glutamicum for selective high-level production of the chiral drug precursor and cell-protective extremolyte L-pipecolic acid.
    Article Snippet: .. The performance of L-pipecolic acid-producing strains was evaluated in a fed-batch process performed in 1 L lab-scale bioreactors, monitored, and controlled using DASGIP control software (SR0700ODLS, Eppendorf, Hamburg, Germany). .. The batch medium (300 mL) contained, per liter: 85 g of glucose, 25 g of (NH4)2SO4, 15 g of yeast extract (Becton Dickinson), 2 g of citric acid, 1.25 g of KH2PO4, 1.25 g of Na2HPO4, 1.25 g of MgSO4 ⋅ 7 H2O, 168 mg of CaSO4 ⋅ 2 H2O, 70 mg of FeSO4 ⋅ 7 H2O, 30 mg of ZnSO4 ⋅ 7 H2O, 9.1 mg of MnSO4 ⋅ H2O, 15 mL of vitamin solution (300 mg L− 1 biotin, 500 mg L− 1 thiamin ⋅ HCl, 600 mg L− 1 nicotinamide, 2 g L− 1 calcium pantothenate), and 1.43 mL of a trace element solution (2.1 g L− 1 citric acid, 228 mg L− 1 CuSO4 ⋅ 5 H2O, 214 mg L− 1 CoSO4 ⋅ 7 H2O, 169 mg L− 1 NiSO4 ⋅ 6 H2O, 150 mg L− 1 Na2B4O7⋅10H2O, and 29.5 mg L− 1 Na2MoO4 ⋅ 2 H2O).

    Antiviral Assay:

    Article Title: Systems metabolic engineering of Corynebacterium glutamicum eliminates all by-products for selective and high-yield production of the platform chemical 5-aminovalerate.
    Article Snippet: .. C. glutamicum was benchmarked for its performance to produce AVA using 1 L lab-scale bioreactors (DASGIP Eppendorf, Jülich, Germany). .. The process was started with 300 mL glucose-based batch medium that contained 80 g of glucose, 25 g of (NH4)2SO4, 15 g of yeast extract (Difco, Becton and Dickinson), 2 g of citrate, 1.25 g of KH2PO4, 1.25 g of Na2HPO4, 1.25 g of MgSO4⋅7 H2O, 165 mg of CaSO4⋅2 H2O, 70 mg of FeSO4⋅7 H2O, 30 mg of ZnSO4⋅7 H2O, 9 mg of MnSO4⋅H2O, 650 μg of CoSO4⋅5 H2O, 600 μg of CoSO4⋅7 H2O, 480 μg of NiSO4⋅6 H2O, 400 μg of boric acid, 85 μg of Na2MoO4⋅2 H2O, 30 mg of calcium pantothenate, 9 mg of nicotine amide, 7.5 mg of thiamine⋅HCl, 3 mg of biotin, and 1 mL of antifoam 204 (Sigma-Aldrich, Steinheim, Germany) per liter.



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    Image Search Results


    Effect of lysE deletion on 5-aminovaleramide production in C. glutamicum. Strains expressing Ptuf davB either episomally in the basic lysine producer LYS-1 (A) or genomically in the advanced producer LYS-12 (B) were compared with their respective lysE-deficient derivatives. The data represent the yields for lysine and 5-AVD and the specific growth rate. Cultivations were performed in a miniaturized bioreactor system with glucose minimal medium. Data represent mean ± standard error from three biological replicates

    Journal: Microbial Cell Factories

    Article Title: Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer

    doi: 10.1186/s12934-026-02922-1

    Figure Lengend Snippet: Effect of lysE deletion on 5-aminovaleramide production in C. glutamicum. Strains expressing Ptuf davB either episomally in the basic lysine producer LYS-1 (A) or genomically in the advanced producer LYS-12 (B) were compared with their respective lysE-deficient derivatives. The data represent the yields for lysine and 5-AVD and the specific growth rate. Cultivations were performed in a miniaturized bioreactor system with glucose minimal medium. Data represent mean ± standard error from three biological replicates

    Article Snippet: Strains were grown in 1 L lab-scale bioreactors, monitored and controlled using the DASGIP control software (SR0700ODLS, Eppendorf, Hamburg, Germany).

    Techniques: Expressing

    A summary of hybrid biotechnological/membrane separation processes and the applied sterilization method. The dashes denote absence of relevant data.

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    Article Title: The Effect of Heat Sterilization on Key Filtration Performance Parameters of a Commercial Polymeric (PVDF) Hollow-Fiber Ultrafiltration Membrane

    doi: 10.3390/membranes12080725

    Figure Lengend Snippet: A summary of hybrid biotechnological/membrane separation processes and the applied sterilization method. The dashes denote absence of relevant data.

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